货号 | EK1588 |
---|---|
产品名称 | Rat WISP1/CCN4 PicoKine ELISA Kit |
产品描述 | For quantitative detection of rat WISP1 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO,T23-N367 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 1.56ng/ml-100ng/ml |
基因名称 | Wisp1 |
基因全名 | WNT1 inducible signaling pathway protein 1 |
Uniprot ID | Q99PP0 |
指标别名 | ducible-signaling pathway protein 1; WISP-1; CCN family member 4; ELM-1; Wisp1; Ccn4, Elm1 |
背景资料 | WISP1(WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1589-CAP | 96-well plate precoated with anti- rat WISP1 antibody | 1 |
EK1589-ST | Lyophilized recombinant rat WISP1 standard | 10ng/tubex2 |
EK1589-DA | Biotinylated anti- rat WISP1 antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 2062 | 13113 | 50481 | 2543 | 13210 | 5100 |
Standard deviation | 88.7 | 760.55 | 3129.8 | 167.8 | 779.39 | 387.6 |
CV(%) | 4.3% | 5.8% | 6.2% | 6.6% | 5.9% | 7.6% |
Concentration(ng/ml) | 0 | 1.56 | 3.13 | 6.25 | 12.5 | 25 | 50 | 100 |
O.D. | 0.084 | 0.225 | 0.323 | 0.462 | 0.792 | 1.291 | 1.970 | 2.548 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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