货号 | EK1916 |
---|---|
产品名称 | Rat VE-Cadherin/CD144 PicoKine ELISA Kit |
产品描述 | For quantitative detection of rat VE-Cadherin/CD144 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, D46 - Q599 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 312pg/ml-20000pg/ml |
基因名称 | Cdh5 |
基因全名 | cadherin 5 |
Uniprot ID | F1M7E5 |
指标别名 | Cadherin 5; Cdh5; |
背景资料 | Cadherin 5, type 2 or VE-cadherin also known as CD144, is a type of cadherin. It is encoded by the human gene CDH5. This gene is mapped to 19p14. VE-cadherin is a classical cadherin from the cadherin superfamily and the gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell–cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1916-CAP | 96-well plate precoated with anti- rat VE-Cadherin/CD144 antibody | 1 |
EK1916-ST | Lyophilized recombinant rat VE-Cadherin/CD144 standard | 20ng/tube×2 |
EK1916-DA | Biotinylated anti- rat VE-Cadherin/CD144 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 399 | 1935 | 8758 | 443 | 2194 | 8947 |
Standard deviation | 21.95 | 123.84 | 507.96 | 27.47 | 112.11 | 653.13 |
CV(%) | 5.5% | 6.4% | 5.8% | 6.2% | 5.1% | 7.3% |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.012 | 0.147 | 0.264 | 0.463 | 0.819 | 1.613 | 2.165 | 2.793 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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