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| 货号 | EK1995 |
|---|---|
| 产品名称 | Rat STC2 PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of rat STC2 in cell culture supernates, serum and plasma (heparin). |
| 免疫原 | NSO, T25-R296 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <50pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | Stc2 |
| 基因全名 | stanniocalcin 2 |
| Uniprot ID | Q9R0K8 |
| 指标别名 | Stanniocalcin-2； STC-2； Stc2 |
| 背景资料 | Stanniocalcin-2 is a protein that in humans is encoded by the STC2 gene. It is mapped to 10q12. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers. STC2 has opposite effects on calcium and phosphate homeostasis as compared to Stc1. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1995-CAP | 96-well plate precoated with anti- rat STC2 antibody | 1 |
| EK1995-ST | Lyophilized recombinant rat STC2 standard | 10ng/tube×2 |
| EK1995-DA | Biotinylated anti- rat STC2 antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 309 | 1116 | 4854 | 311 | 1189 | 4887 |
| Standard deviation | 12.98 | 50.22 | 276.68 | 15.24 | 68.96 | 307.88 |
| CV(%) | 4.2% | 4.5% | 5.7% | 4.9% | 5.8% | 6.3% |
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.004 | 0.105 | 0.237 | 0.472 | 0.729 | 1.009 | 1.652 | 2.267 |
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