货号 | EK1741 |
---|---|
产品名称 | Rat Serpin A12 PicoKine ELISA Kit |
产品描述 | For quantitative detection of rat Serpin A12 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, L21-P411 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | Serpina12 |
基因全名 | serpin family A member 12 |
Uniprot ID | Q8R4Z1 |
指标别名 | Serpin A12; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin; Serpina12 |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1741-CAP | 96-well plate precoated with anti- rat Serpin A12 antibody | 1 |
EK1741-ST | Lyophilized recombinant rat Serpin A12 standard | 10ng/tube×2 |
EK1741-DA | Biotinylated anti- rat Serpin A12 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 18 | 79 | 426 | 25 | 86 | 439 |
Standard deviation | 0.86 | 4.50 | 19.60 | 1.28 | 5.07 | 28.10 |
CV(%) | 4.8% | 5.7% | 4.6% | 5.1% | 5.9% | 6.4% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.008 | 0.087 | 0.171 | 0.295 | 0.574 | 1.100 | 1.981 | 2.761 |
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