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| 货号 | EK2055 |
|---|---|
| 产品名称 | Rat SEMA4G PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of rat SEMA4G in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
| 免疫原 | NSO, V18-M673 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 15.6pg/ml-1000pg/ml |
| 基因名称 | Sema4g |
| 基因全名 | semaphorin 4G |
| Uniprot ID | D4A6G2 |
| 指标别名 | Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (Semaphorin) 4G (Predicted); Semaphorin 4G; Sema4g; Sema4g_predicted; rCG_57705 |
| 背景资料 | Semaphorin-4G is a protein that in humans is encoded by the SEMA4G gene. This gene is mapped to 1q54. Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphoring (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene and the gene for mitochondrial ribosomal protein L43 overlap at map location 10q24.31 and are transcribed in opposite directions. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK2055-CAP | 96-well plate precoated with anti- rat SEMA4G antibody | 1 |
| EK2055-ST | Lyophilized recombinant rat SEMA4G standard | 10ng/tube×2 |
| EK2055-DA | Biotinylated anti- rat SEMA4G antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 20 | 95 | 467 | 25 | 121 | 479 |
| Standard deviation | 0.86 | 4.94 | 21.48 | 1.53 | 7.14 | 35.45 |
| CV(%) | 4.3% | 5.2% | 4.6% | 6.1% | 5.9% | 7.4% |
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.026 | 0.162 | 0.275 | 0.454 | 0.773 | 1.402 | 1.963 | 2.253 |
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