货号 | EK1735 |
---|---|
产品名称 | Rat SEMA3F PicoKine ELISA Kit |
产品描述 | For quantitative detection of rat SEMA3F in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | NSO, A19-T785 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | Plxna3 |
基因全名 | semaphorin 3F |
Uniprot ID | D3ZPX4 |
指标别名 | Plexin-A3; Plxna3 |
背景资料 | Semaphorin-3F is a protein that in humans is encoded by the SEMA3F gene. It is mapped to 8q32. The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues. Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin 3 family, consisting of human semaphorins 3A-G, chicken collapsin, and mouse semaphorins 3A-G, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones through an interaction with a collapsin response mediator protein of relative molecular mass 62K , a putative homolog of an axonal guidance associated UNC33 gene product. SEMA3F is a secreted member of the semaphorin III family. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1735-CAP | 96-well plate precoated with anti- rat SEMA3F antibody | 1 |
EK1735-ST | Lyophilized recombinant rat SEMA3F standard | 10ng/tube×2 |
EK1735-DA | Biotinylated anti- rat SEMA3F antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 58 | 227 | 919 | 59 | 236 | 947 |
Standard deviation | 3.02 | 12.03 | 43.19 | 3.60 | 17.46 | 48.30 |
CV(%) | 5.2% | 5.3% | 4.7% | 6.1% | 7.4% | 5.1% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.006 | 0.079 | 0.117 | 0.214 | 0.403 | 0.701 | 1.218 | 2.037 |
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