货号 | EK1883 |
---|---|
产品名称 | Rat Plexin B2 PicoKine ELISA Kit |
产品描述 | For quantitative detection of rat Plexin B2 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | CHO, L20-P1202 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 0.78ng/ml-50ng/ml |
基因名称 | Plxnb2 |
基因全名 | plexin B2 |
Uniprot ID | D3ZQ57 |
指标别名 | Plexin B2; Plxnb2; rCG_59297 |
背景资料 | Plexin-B2 is a protein that in humans is encoded by the PLXNB2 gene. It is mapped to 7q34. Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1883-CAP | 96-well plate precoated with anti- rat Plexin B2 antibody | 1 |
EK1883-ST | Lyophilized recombinant rat Plexin B2 standard | 10ng/tube×2 |
EK1883-DA | Biotinylated anti- rat Plexin B2 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 31 | 190 | 449 | 33 | 201 | 456 |
Standard deviation | 1.89 | 10.26 | 19.76 | 1.55 | 14.67 | 35.11 |
CV(%) | 6.1% | 5.4% | 4.4% | 4.7% | 7.3% | 7.7% |
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.126 | 0.183 | 0.235 | 0.344 | 0.536 | 0.917 | 1.513 | 2.230 |
典型操作程序:
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知,且每位咨询者购买情况、提问时间等不同,为此以下回复仅对提问者3天内有效,其他网友仅供参考!若由此给您带来不便请多多谅解,谢谢!
服务热线
0771-3293894