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| 货号 | EK1855 |
|---|---|
| 产品名称 | Rat PDGF-CC PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of rat PDGF-CC in cell culture supernates, serum and plasma (heparin, EDTA). |
| 免疫原 | E.coli, A235-G345 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 15.6pg/ml-1000pg/ml |
| 基因名称 | Pdgfc |
| 基因全名 | platelet derived growth factor C |
| Uniprot ID | Q9EQX6 |
| 指标别名 | Platelet-derived growth factor C; PDGF-C; Fallotein; Spinal cord-derived growth factor; SCDGF; VEGF-E; Platelet-derived growth factor C, latent form; PDGFC latent form; Platelet-derived growth factor C, receptor-binding form; PDGFC receptor-binding form; Pdgfc; Scdgf |
| 背景资料 | Platelet-derived growth factor C, also known as PDGF-C, is a 345-amino acid protein that in humans is encoded by the PDGFC gene. It is mapped to 2q33-q34. Platelet-derived growth factors are important in connective tissue growth, survival and function, and consist of disulphide-linked dimers involving two polypeptide chains, PDGF-A and PDGF-B. PDGF-C is a member of the PDGF/VEGF family of growth factors with a unique two-domain structure and expression pattern. PDGF-C was not previously identified with PDGF-A and PDGF-B, possibly because it may be that it is synthesized and secreted as a latent growth factor, requiring proteolytic removal of the N-terminal CUB domain for receptor binding and activation. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1855-CAP | 96-well plate precoated with anti- rat PDGF-CC antibody | 1 |
| EK1855-ST | Lyophilized recombinant rat PDGF-CC standard | 10ng/tube×2 |
| EK1855-DA | Biotinylated anti- rat PDGF-CC antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 16 | 113 | 464 | 23 | 117 | 472 |
| Standard deviation | 0.64 | 5.31 | 24.13 | 1.17 | 6.90 | 33.51 |
| CV(%) | 4.0% | 4.7% | 5.2% | 5.1% | 5.9% | 7.1% |
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.032 | 0.085 | 0.143 | 0.248 | 0.423 | 0.816 | 1.359 | 2.231 |
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