货号 | EK1698 |
---|---|
产品名称 | Rat PDGF-BB PicoKine ELISA Kit |
产品描述 | For quantitative detection of Rat PDGF-BB in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli,S82-T190 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | Tgfb1 |
基因全名 | transforming growth factor, beta 1 |
Uniprot ID | Q62908 |
指标别名 | Cysteine and glycine-rich protein 2; Cysteine-rich protein 2; CRP2; Smooth muscle cell LIM protein; SmLIM; Csrp2; Smlim |
背景资料 | PDGF-BB encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1698-CAP | 96-well plate precoated with anti- rat PDGF-BB antibody | 1 |
EK1698-ST | Lyophilized recombinant rat PDGF-BB standard | 10ng/tubex2 |
EK1698-DA | Biotinylated anti- rat PDGF-BB antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 108 | 651 | 1071 | 121 | 714 | 1110 |
Standard deviation | 4.43 | 33.85 | 58.9 | 7.6 | 52.84 | 86.58 |
CV(%) | 4.1% | 5.2% | 5.5% | 6.3% | 7.4% | 7.8% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.066 | 0.045 | 0.129 | 0.198 | 0.478 | 0.991 | 1.817 | 2.424 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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