0
| 货号 | EK1701 |
|---|---|
| 产品名称 | Rat PCSK9 PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of rat PCSK9 in cell culture supernates, serum and plasma (heparin, EDTA). |
| 免疫原 | NSO,S156-Q694 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 62.5pg/ml-4000pg/ml |
| 基因名称 | Pcsk9 |
| 基因全名 | proprotein convertase subtilisin/kexin type 9 |
| Uniprot ID | P59996 |
| 指标别名 | Proprotein convertase subtilisin/kexin type 9; Neural apoptosis-regulated convertase 1; NARC-1; Proprotein convertase 9; PC9; Subtilisin/kexin-like protease PC9; Pcsk9; Narc1 |
| 背景资料 | PCSK9 is the 9th member of the proprotein convertase family of proteins that activate other proteins.PCSK9 has medical importance because it acts in lipoprotein homeostasis. PCSK9 is ubiquitously expressed in many tissues and cell types. PCSK9 binds to the receptor for low-density lipoprotein particles (LDL), which typically transport 3,000 to 6,000 fat molecules (including cholesterol) per particle, within extracellular fluid. The LDL receptor (LDLR), on liver and other cell membranes, binds and initiates ingestion of LDL-particles from extracellular fluid into cells, thus reducing LDL particle concentrations. |
| 显色时间 | 25-30 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1701-CAP | 96-well plate precoated with anti- rat PCSK9 antibody | 1 |
| EK1701-ST | Lyophilized recombinant rat PCSK9 standard | 4ng/tubex2 |
| EK1701-DA | Biotinylated anti- rat PCSK9 antibody | 130ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 140 | 555 | 1161 | 145 | 563 | 1332 |
| Standard deviation | 6.58 | 30.53 | 68.5 | 9.28 | 37.72 | 99.9 |
| CV(%) | 4.7% | 5.5% | 5.9% | 6.4% | 6.7% | 7.5% |
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.061 | 0.122 | 0.175 | 0.276 | 0.520 | 0.952 | 1.408 | 2.428 |
1：准备试剂和标准品
2：每孔添加100ul样品和标准品，37℃反应90分钟，不洗。
3：每孔添加100ul生物素标记抗体，37℃反应60分钟。
4：0.01M TBS洗涤3次。
5：每孔添加100ulABC，37℃反应30分钟。
6：0.01M TBS洗涤5次。
7：TMB37℃避光反应30分钟以内。
8：加入TMB终止液，读数。
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知，且每位咨询者购买情况、提问时间等不同，为此以下回复仅对提问者3天内有效，其他网友仅供参考！若由此给您带来不便请多多谅解，谢谢！
服务热线
0771-3293894
新浪微博
腾讯微博
QQ空间
客服
