货号 | EK1594 |
---|---|
产品名称 | Rat P-Cadherin PicoKine ELISA Kit |
产品描述 | For quantitative detection of rat P-Cadherin in cell serum. |
免疫原 | NSO,E100-G647 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <5pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | Cdh3 |
基因全名 | cadherin 3 |
Uniprot ID | F1LMI3 |
指标别名 | Cadherin 3 |
背景资料 | Cadherins are calcium-dependent cell-cell adhesion molecules that mediate cell-cell binding in a homophilic manner. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherin expression is regulated spatially as well as temporally. Cadherins are thought to play an important role in development and maintenance of tissues through selective cell-cell adhesion activity and may be involved also in the invasion and metastasis of malignant tumors. Cadherin regulates dendritic spine morphogenesis. A cadherin gene cluster is mapped to a region of chromosome 5 subject to frequent allelic loss in carcinoma. The standard product used in this kit is recombinant P-Cadherin with the molecular mass of 120-130Kda after glycosylation. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1594-CAP | 96-well plate precoated with anti- rat P-Cadherin antibody | 1 |
EK1594-ST | Lyophilized recombinant rat P-Cadherin standard | 10ng/tubex2 |
EK1594-DA | Biotinylated anti- rat P-Cadherin antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 28 | 221 | 384 | 30 | 229 | 407 |
Standard deviation | 1.18 | 9.95 | 19.58 | 1.77 | 14.66 | 30.5 |
CV(%) | 4.2% | 4.5% | 5.1% | 5.9% | 6.4% | 7.5% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.094 | 0.224 | 0.324 | 0.514 | 0.748 | 1.063 | 1.533 | 2.078 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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