货号 | EK2016 |
---|---|
产品名称 | Rat Midkine PicoKine ELISA Kit |
产品描述 | For quantitative detection of rat Midkine in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | E.coli, V21-D140 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | Mdk |
基因全名 | midkine |
Uniprot ID | Q9R1S9 |
指标别名 | Midkine; MK; Mdk |
背景资料 | Midkine (MK) is a member of a family of neurotrophic and developmentally-regulated heparin-binding molecules that currently consists of midkine, pleiotrophin/PTN and avian RI-HB. This gene is mapped to 3q24. Across species, MK shows 87% amino acid identity between the human and mouse proteins. Between family members, human MK is approximately 50% identical to human PTN. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2016-CAP | 96-well plate precoated with anti- rat Midkine antibody | 1 |
EK2016-ST | Lyophilized recombinant rat Midkine standard | 10ng/tube×2 |
EK2016-DA | Biotinylated anti- rat Midkine antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 25 | 109 | 457 | 30 | 115 | 486 |
Standard deviation | 1.20 | 5.45 | 21.02 | 1.53 | 6.79 | 31.10 |
CV(%) | 4.8% | 5.0% | 4.6% | 5.1% | 5.9% | 6.4% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.027 | 0.111 | 0.195 | 0.346 | 0.614 | 1.040 | 1.697 | 2.255 |
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