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Rat MAG PicoKine ELISA Kit 96T

价:
2800.00
价:
¥2800.00

号:EK2062

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

客服 郭恒 蔡玉坤 曾宪飞 技术咨询

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Product Brief

  • 产品概况

    货号 EK2062
    产品名称 Rat MAG PicoKine ELISA Kit
    产品描述 For quantitative detection of rat MAG in cell culture supernates, serum and plasma (heparin, EDTA, citrate).
    免疫原 NSO, G20-P516
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <50pg/ml
    检测范围 78pg/ml-5000pg/ml
    基因名称 Mag
    基因全名 myelin-associated glycoprotein
    Uniprot ID P07722
    指标别名 Myelin-associated glycoprotein; 1B236; Brain neuron cytoplasmic protein 3; Sialic acid-binding Ig-like lectin 4a; Siglec-4a; Mag
    背景资料 Myelin-associated glycoprotein (MAG, Siglec-4) is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NoGo-66 receptor(NgR). This gene is mapped to 1q21. The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.
    显色时间 15-20

    试剂盒内容

    SKU 名称 数量
    Catalog number Description Quantity
    EK2062-CAP 96-well plate precoated with anti- rat MAG antibody 1
    EK2062-ST Lyophilized recombinant rat MAG standard 10ng/tube×2
    EK2062-DA Biotinylated anti- rat MAG antibody 130μl(dilution 1:100)
    AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100)
    AR1106-1 Sample diluent buffer 30ml
    AR1106-2 Antibody diluent buffer 12ml
    AR1106-3 ABC diluent buffer 12ml
    AR1104 TMB color developing agent 10ml
    AR1105 TMB stop solution 10ml
    PLA-SEA Adhesive cover 4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mean(pg/ml) 95 610 2357 108 613 2474
    Standard deviation 4.28 26.23 120.21 7.13 42.30 180.60
    CV(%) 4.5% 4.3% 5.1% 6.6% 6.9% 7.3%

    Rat MAG PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20)
    Concentration(pg/ml) 0 78 156 312 625 1250 2500 5000
    O.D. 0.055 0.125 0.169 0.299 0.545 0.956 1.437 2.064
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

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