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| 货号 | EK1841 |
|---|---|
| 产品名称 | Rat JAM-C PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of rat JAM-C in cell culture supernates, serum and plasma (heparin, EDTA). |
| 免疫原 | NSO, V32-N241 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <5pg/ml |
| 检测范围 | 15.6pg/ml-1000pg/ml |
| 基因名称 | Jam3 |
| 基因全名 | junctional adhesion molecule 3 |
| Uniprot ID | Q68FQ2 |
| 指标别名 | Junctional adhesion molecule C; JAM-C; Junctional adhesion molecule 3; JAM-3; Jam3 |
| 背景资料 | JAM3 belongs to the junctional adhesion molecule protein family and functions as a receptor for another member of this family. This gene is mapped to 8q13. JAM3 plays a part in cell-cell adhesion. The soluble form of JAM3 is a mediator of angiogenesis. JAM3 is a counter-receptor for ITGAM, mediating leukocyteplatelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). A mutation in an intron of the JAM3 gene is linked with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1841-CAP | 96-well plate precoated with anti- rat JAM-C antibody | 1 |
| EK1841-ST | Lyophilized recombinant rat JAM-C standard | 10ng/tube×2 |
| EK1841-DA | Biotinylated anti- rat JAM-C antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 30 | 159 | 382 | 31 | 163 | 472 |
| Standard deviation | 1.29 | 7.31 | 21.77 | 1.92 | 10.27 | 34.93 |
| CV(%) | 4.3% | 4.6% | 5.7% | 6.2% | 6.3% | 7.4% |
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.039 | 0.108 | 0.186 | 0.321 | 0.599 | 1.017 | 1.586 | 2.200 |
1：准备试剂和标准品
2：每孔添加100ul样品和标准品，37℃反应90分钟，不洗。
3：每孔添加100ul生物素标记抗体，37℃反应60分钟。
4：0.01M TBS洗涤3次。
5：每孔添加100ulABC，37℃反应30分钟。
6：0.01M TBS洗涤5次。
7：TMB37℃避光反应30分钟以内。
8：加入TMB终止液，读数。
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