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| 货号 | EK1742 |
|---|---|
| 产品名称 | Rat IL6ST PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of rat IL6ST in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
| 免疫原 | NSO, Q23-E618 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <0.05ng/ml |
| 检测范围 | 0.78ng/ml-50ng/ml |
| 基因名称 | Il6st |
| 基因全名 | interleukin 6 signal transducer |
| Uniprot ID | P40190 |
| 指标别名 | Interleukin-6 receptor subunit beta; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; Oncostatin-M receptor subunit alpha; CD130; Il6st |
| 背景资料 | GP130(Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membranebound gp130. Gp130 is an important part of many different types of signaling complexes. Gp130 molecules with mutations in either of these segments could not transduce a growth signal. |
| 显色时间 | 25-30 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1742-CAP | 96-well plate precoated with anti- rat IL6ST antibody | 1 |
| EK1742-ST | Lyophilized recombinant rat IL6ST standard | 50ng/tube×2 |
| EK1742-DA | Biotinylated anti- rat IL6ST antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1254 | 4579 | 20188 | 1322 | 5546 | 21456 |
| Standard deviation | 66.46 | 290.3 | 847.9 | 99.15 | 321.7 | 1652.1 |
| CV(%) | 5.3% | 6.1% | 4.2% | 7.5% | 5.8% | 7.7% |
| Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.008 | 0.329 | 0.516 | 0.817 | 1.102 | 1.336 | 1.568 | 1.839 |
1：准备试剂和标准品
2：每孔添加100ul样品和标准品，37℃反应90分钟，不洗。
3：每孔添加100ul生物素标记抗体，37℃反应60分钟。
4：0.01M TBS洗涤3次。
5：每孔添加100ulABC，37℃反应30分钟。
6：0.01M TBS洗涤5次。
7：TMB37℃避光反应30分钟以内。
8：加入TMB终止液，读数。
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