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| 货号 | EK1624 |
|---|---|
| 产品名称 | Rat HE4/WFDC2 PicoKine ELISA Kit |
| 产品描述 | For Quantitative Detection of rat HE4 in cell culture supernates, serum and plasma (heparin, EDTA). |
| 免疫原 | E.coli,E31-F168 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | Wfdc2 |
| 基因全名 | WAP four-disulfide core domain 2 |
| Uniprot ID | Q8CHN3 |
| 指标别名 | WAP four-disulfide core domain protein 2; Epididymal secretory protein 4; RE4; Wfdc2 |
| 背景资料 | WAP four-disulfide core domain protein 2, also known as Human Epididymis Protein 4 (HE4), is a protein that in humans is encoded by the WFDC2 gene. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1624-CAP | 96-well plate precoated with anti- rat HE4 antibody | 1 |
| EK1624-ST | Lyophilized recombinant rat HE4 standard | 10ng/tube×2 |
| EK1624-DA | Biotinylated anti- rat HE4 antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 295 | 1986 | 4547 | 316 | 2068 | 4341 |
| Standard deviation | 16.22 | 129.09 | 227.35 | 19.9 | 169.57 | 273.48 |
| CV(%) | 5.5% | 6.5% | 5% | 6.3% | 8.2% | 6.3% |
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.082 | 0.173 | 0.225 | 0.311 | 0.496 | 0.819 | 1.297 | 2.064 |
典型操作程序：
1?准备试剂和标准品
2?每孔添加100ul样品和标准品，37℃反应90分钟，不洗。
3?每孔添加100ul生物素标记抗体，37℃反应60分钟。
4?0.01M TBS洗涤3次。
5?每孔添加100ulABC，37℃反应30分钟。
6?0.01M TBS洗涤5次。
7?TMB37℃避光反应30分钟以内。
8?加入TMB终止液，读数。
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