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| 货号 | EK2071 |
|---|---|
| 产品名称 | Rat GFRA1 PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of rat GFRA1 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
| 免疫原 | NSO, D25-S430 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 15.6pg/ml-1000pg/ml |
| 基因名称 | Gfra1 |
| 基因全名 | GDNF family receptor alpha 1 |
| Uniprot ID | Q62997 |
| 指标别名 | GDNF family receptor alpha-1; GDNF receptor alpha-1; GDNFR-alpha-1; GFR-alpha-1; RET ligand 1; TGF-beta-related neurotrophic factor receptor 1; Gfra1; Gdnfra; Retl1; Trnr1 |
| 背景资料 | GDNF family receptor alpha-1 (GFRα1), also known as the GDNF receptor, is a protein that in humans is encoded by the GFRA1 gene. It is mapped to 1q55. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.GDNF family receptor alpha 1 has been shown to interact with GDNF[8][9] and RET proto-oncogene. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK2071-CAP | 96-well plate precoated with anti- rat GFRA1 antibody | 1 |
| EK2071-ST | Lyophilized recombinant rat GFRA1 standard | 10ng/tube×2 |
| EK2071-DA | Biotinylated anti- rat GFRA1 antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 22 | 99 | 314 | 28 | 121 | 415 |
| Standard deviation | 1.14 | 5.25 | 17.27 | 1.85 | 8.23 | 32.37 |
| CV(%) | 5.2% | 5.3% | 5.5% | 6.6% | 6.8% | 7.8% |
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.039 | 0.130 | 0.242 | 0.507 | 0.824 | 1.311 | 1.776 | 2.194 |
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