货号 | EK1915 |
---|---|
产品名称 | Rat GAS6 PicoKine ELISA Kit |
产品描述 | For quantitative detection of rat GAS6 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, D115-P673 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | GAS6 |
基因全名 | growth arrest specific 6 |
Uniprot ID | Q14393 |
指标别名 | Growth arrest-specific protein 6; GAS-6; AXL receptor tyrosine kinase ligand; GAS6; AXLLG |
背景资料 | GAS6(Growth arrest-specific 6), also known as AXLLG or AXSF, is a human gene coding for the Gas6 protein. It is mapped to 13q34. GAS6 was originally found as a gene upregulated by growth arrested fibroblasts. This gene product is a gamma-carboxyglutamic acid(Gla)-containing protein which thought to be involved in the stimulation of cell proliferation, and may play a role in thrombosis. What's more, the product of the GAS6 gene, a ligand for the AXL, MER, and TYRO3 tyrosine kinase receptors, is a vitamin K-dependent protein, structurally related to anticoagulant protein S but lacking its anticoagulant activity. This protein also shows great importance in the cardiovascular system. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1915-CAP | 96-well plate precoated with anti- rat GAS6 antibody | 1 |
EK1915-ST | Lyophilized recombinant rat GAS6 standard | 10ng/tube×2 |
EK1915-DA | Biotinylated anti- rat GAS6 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 73 | 308 | 856 | 75 | 321 | 969 |
Standard deviation | 3.94 | 13.24 | 48.79 | 4.65 | 20.22 | 73.64 |
CV(%) | 5.4% | 4.3% | 5.7% | 6.2% | 6.3% | 7.6% |
Concentration(pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.042 | 0.115 | 0.186 | 0.336 | 0.598 | 1.116 | 1.898 | 2.604 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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