货号 | EK1589 |
---|---|
产品名称 | Rat Galectin-1 PicoKine ELISA Kit |
产品描述 | For quantitative detection of rat Galectin-1 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli,A2-E135 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | Lgals1 |
基因全名 | galectin 1 |
Uniprot ID | P11762 |
指标别名 | Galectin-1; Gal-1; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; RL 14.5; S-Lac lectin 1; Lgals1 |
背景资料 | Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Its expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. Additionally, Galectin-1 is thought to play a role in creating immune tolerance in pregnancy. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1588-CAP | 96-well plate precoated with anti- rat Galectin-1 antibody | 1 |
EK1588-ST | Lyophilized recombinant rat Galectin-1 standard | 10ng/tubex2 |
EK1588-DA | Biotinylated anti- rat Galectin-1 antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 243 | 1504 | 5437 | 262 | 1512 | 5561 |
Standard deviation | 15.31 | 85.71 | 260.98 | 17.29 | 110.4 | 428.2 |
CV(%) | 6.3% | 5.7% | 4.8% | 6.6% | 7.3% | 7.7% |
Concentration(pg/ml) | 0 | 156 | 313 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.079 | 0.174 | 0.258 | 0.407 | 0.684 | 1.056 | 1.570 | 2.274 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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