货号 | EK2101 |
---|---|
产品名称 | Rat Flt-3 ligand PicoKine ELISA Kit |
产品描述 | For quantitative detection of rat Flt-3 ligand in cell culture supernates and serum. |
免疫原 | NSO, G27-R188 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | Flt3lg |
基因全名 | fms-related tyrosine kinase 3 ligand |
Uniprot ID | A0A1W2Q6J3 |
指标别名 | Fms-related tyrosine kinase 3 ligand; Flt3lg |
背景资料 | Fms-related tyrosine kinase 3 ligand (FLT3LG) is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 1q22. Flt3 ligand (FL) is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt3 ligand stimulates the proliferation and differentiation of various blood cell progenitors. For example, it is a major growth factor stimulating the growth of dendritic cells. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2101-CAP | 96-well plate precoated with anti- rat Flt-3 ligand antibody | 1 |
EK2101-ST | Lyophilized recombinant rat Flt-3 ligand standard | 10ng/tube×2 |
EK2101-DA | Biotinylated anti- rat Flt-3 ligand antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 36 | 160 | 699 | 41 | 184 | 759 |
Standard deviation | 1.62 | 6.88 | 28.66 | 2.46 | 10.67 | 49.34 |
CV(%) | 4.5% | 4.3% | 4.1% | 6.0% | 5.8% | 6.5% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.030 | 0.117 | 0.199 | 0.323 | 0.590 | 0.939 | 1.654 | 2.280 |
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