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| 货号 | EK1695 |
|---|---|
| 产品名称 | Rat FGFBP1 PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of rat FGFBP1 in cell culture supernates, serum and plasma (heparin, EDTA). |
| 免疫原 | NSO,E21-C238 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 78pg/ml-5000pg/ml |
| 基因名称 | Fgfbp1 |
| 基因全名 | fibroblast growth factor binding protein 1 |
| Uniprot ID | Q9QY10 |
| 指标别名 | Fibroblast growth factor-binding protein 1; FGF-BP; FGF-BP1; FGF-binding protein 1; FGFBP-1; Growth factor-binding protein 1; Fgfbp1 |
| 背景资料 | Fibroblast growth factor-binding protein 1 is a protein that in humans is encoded by the FGFBP1 gene. This gene encodes a secreted fibroblast growth factor carrier protein. The encoded protein plays a critical role in cell proliferation, differentiation and migration by binding to fibroblast growth factors and potentiating their biological effects on target cells. The encoded protein may also play a role in tumor growth as an angiogenic switch molecule, and expression of this gene has been associated with several types of cancer including pancreatic and colorectal adenocarcinoma. A pseudogene of this gene is also located on the short arm of chromosome 5. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1695-CAP | 96-well plate precoated with anti- rat FGFBP1 antibody | 1 |
| EK1695-ST | Lyophilized recombinant rat FGFBP1 standard | 10ng/tubex2 |
| EK1695-DA | Biotinylated anti- rat FGFBP1 antibody | 130ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 112 | 566 | 2662 | 119 | 607 | 2774 |
| Standard deviation | 6.9 | 36.79 | 114.47 | 6.19 | 37.03 | 199.7 |
| CV(%) | 6.2% | 6.5% | 4.3% | 5.2% | 6.1% | 7.2% |
| Concentration(pg/ml) | 0 | 78 | 156 | 313 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.066 | 0.156 | 0.211 | 0.336 | 0.565 | 0.991 | 1.741 | 2.475 |
1：准备试剂和标准品
2：每孔添加100ul样品和标准品，37℃反应90分钟，不洗。
3：每孔添加100ul生物素标记抗体，37℃反应60分钟。
4：0.01M TBS洗涤3次。
5：每孔添加100ulABC，37℃反应30分钟。
6：0.01M TBS洗涤5次。
7：TMB37℃避光反应30分钟以内。
8：加入TMB终止液，读数。
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