货号 | EK1591 |
---|---|
产品名称 | Rat FGF23 PicoKine ELISA Kit |
产品描述 | For quantitative detection of rat FGF23 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli,Y25-V251 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | Fgf23 |
基因全名 | fibroblast growth factor 23 |
Uniprot ID | Q8VI82 |
指标别名 | Fibroblast growth factor 23; FGF-23; Fgf23 |
背景资料 | Fibroblast growth factor 23 or FGF23 is a protein that in humans is encoded by the FGF23 gene. It is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 is secreted by Osteocytes in response to elevated Calcitriol. And it acts on the kidneys, where it decreases the expression of NPT2, a sodium-phosphate cotransporter in the proximal tubule. Thus, FGF23 decreases the reabsorption and increases excretion of phosphate. Also, FGF23 may suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1591-CAP | 96-well plate precoated with anti- rat FGF23 antibody | 1 |
EK1591-ST | Lyophilized recombinant rat FGF23 standard | 10ng/tubex2 |
EK1591-DA | Biotinylated anti- rat FGF23 antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 55 | 244 | 925 | 59 | 248 | 978 |
Standard deviation | 2.81 | 13.18 | 39.88 | 2.9 | 16.6 | 77.3 |
CV(%) | 5.1% | 5.4% | 4.3% | 4.9% | 6.7% | 7.9% |
Concentration(pg/ml) | 0.0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.108 | 0.236 | 0.303 | 0.655 | 0.762 | 1.201 | 1.735 | 2.091 |
典型操作程序:
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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