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| 货号 | EK1595 |
|---|---|
| 产品名称 | Rat FGF21 PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of rat FGF21 in cell culture supernates, serum and plasma (heparin, EDTA). |
| 免疫原 | E.coli,Y30-S208 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 31.2pg/ml-2000pg/ml |
| 基因名称 | Fgf21 |
| 基因全名 | fibroblast growth factor 21 |
| Uniprot ID | Q8VI80 |
| 指标别名 | Fibroblast growth factor |
| 背景资料 | Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al.(2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1595-CAP | 96-well plate precoated with anti- rat FGF21 antibody | 1 |
| EK1595-ST | Lyophilized recombinant rat FGF21 standard | 10ng/tubex2 |
| EK1595-DA | Biotinylated anti- rat FGF21 antibody | 130ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 50 | 155 | 638 | 57 | 172 | 749 |
| Standard deviation | 2.10 | 6.67 | 30.62 | 2.79 | 11.01 | 58.42 |
| CV(%) | 4.2% | 4.3% | 4.8% | 4.9% | 6.4% | 7.8% |
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.071 | 0.144 | 0.207 | 0.352 | 0.586 | 0.956 | 1.569 | 2.251 |
1：准备试剂和标准品
2：每孔添加100ul样品和标准品，37℃反应90分钟，不洗。
3：每孔添加100ul生物素标记抗体，37℃反应60分钟。
4：0.01M TBS洗涤3次。
5：每孔添加100ulABC，37℃反应30分钟。
6：0.01M TBS洗涤5次。
7：TMB37℃避光反应30分钟以内。
8：加入TMB终止液，读数。
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