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| 货号 | EK1623 |
|---|---|
| 产品名称 | Rat FABP3 PicoKine ELISA Kit |
| 产品描述 | For Quantitative Detection of rat FABP3 in cell culture supernates, serum and plasma (heparin, EDTA). |
| 免疫原 | E.coli,A2-A133 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 93.7pg/ml-6000pg/ml |
| 基因名称 | Fabp3 |
| 基因全名 | fatty acid binding protein 3 |
| Uniprot ID | P07483 |
| 指标别名 | Fatty acid-binding protein, heart; Fatty acid-binding protein 3; Heart-type fatty acid-binding protein; H-FABP; Fabp3 |
| 背景资料 | Heart-type fatty acid binding protein (hFABP), also known as mammary-derived growth inhibitor, is a protein that in humans is encoded by the FABP3 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is also a candidate tumor suppressor gene for human breast cancer. Cardiac-type fatty acid-binding protein (cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP. |
| 显色时间 | 20-25 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1623-CAP | 96-well plate precoated with anti- rat FABP3 antibody | 1 |
| EK1623-ST | Lyophilized recombinant rat FABP3 standard | 10ng/tube×2 |
| EK1623-DA | Biotinylated anti- rat FABP3 antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 131 | 866 | 2555 | 124 | 868 | 2555 |
| Standard deviation | 6.02 | 54.55 | 158.41 | 7.19 | 60.76 | 181.4 |
| CV(%) | 4.6% | 6.3% | 6.2% | 5.8% | 7% | 7.1% |
| Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.075 | 0.166 | 0.229 | 0.356 | 0.588 | 1.016 | 1.722 | 2.411 |
典型操作程序：
1?准备试剂和标准品
2?每孔添加100ul样品和标准品，37℃反应90分钟，不洗。
3?每孔添加100ul生物素标记抗体，37℃反应60分钟。
4?0.01M TBS洗涤3次。
5?每孔添加100ulABC，37℃反应30分钟。
6?0.01M TBS洗涤5次。
7?TMB37℃避光反应30分钟以内。
8?加入TMB终止液，读数。
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