货号 | EK1800 |
---|---|
产品名称 | Rat EphA4 PicoKine ELISA Kit |
产品描述 | For quantitative detection of rat EphA4 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, V20-T547 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | Epha4 |
基因全名 | Eph receptor A4 |
Uniprot ID | D3ZZK3 |
指标别名 | Eph receptor A4; Epha4 |
背景资料 | EPH receptor A4 (ephrin type-A receptor 4) is a protein that in humans is encoded by the EPHA4 gene. It is mapped to 9q33. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1800-CAP | 96-well plate precoated with anti- rat EphA4 antibody | 1 |
EK1800-ST | Lyophilized recombinant rat EphA4 standard | 10ng/tube×2 |
EK1800-DA | Biotinylated anti- rat EphA4 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 47 | 150 | 534 | 56 | 224 | 767 |
Standard deviation | 2.77 | 6.45 | 21.89 | 3.70 | 12.99 | 58.29 |
CV(%) | 5.9% | 4.3% | 4.1% | 6.6% | 5.8% | 7.6% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.032 | 0.175 | 0.294 | 0.511 | 0.851 | 1.320 | 1.825 | 2.213 |
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