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Qiagen 凯杰 Biosharp Omega

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Rat DNER PicoKine ELISA Kit 96T

价:
2800.00
价:
¥2800.00

号:EK2082

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

客服 郭恒 蔡玉坤 曾宪飞 技术咨询

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Product Brief

  • 产品概况

    货号 EK2082
    产品名称 Rat DNER PicoKine ELISA Kit
    产品描述 For quantitative detection of rat DNER in cell culture supernates, serum and plasma (heparin, EDTA, citrate).
    免疫原 NSO, A26-Y649
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <50pg/ml
    检测范围 312pg/ml-20000pg/ml
    基因名称 Dner
    基因全名 delta/notch-like EGF repeat containing
    Uniprot ID Q5U215
    指标别名 Delta/notch-like EGF repeat containing; Dner
    背景资料 Delta and Notch-like epidermal growth factor-related receptor is a protein that in humans is encoded by the DNER gene. It is mapped to 9q35. DNER, also known as BET, is a type I transmembrane glycoprotein that is specifically expressed on nonaxonal areas of post-mitotic neurons. The protein has an extracellular domain containing ten distinct EGF-like repeats similar to those found on Delta and Notch. Human and mouse DNER share 90% amino acid sequence identity.
    显色时间 15-20

    试剂盒内容

    SKU 名称 数量
    Catalog number Description Quantity
    EK2082-CAP 96-well plate precoated with anti- rat DNER antibody 1
    EK2082-ST Lyophilized recombinant rat DNER standard 20ng/tube×2
    EK2082-DA Biotinylated anti- rat DNER antibody 130μl(dilution 1:100)
    AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100)
    AR1106-1 Sample diluent buffer 30ml
    AR1106-2 Antibody diluent buffer 12ml
    AR1106-3 ABC diluent buffer 12ml
    AR1104 TMB color developing agent 10ml
    AR1105 TMB stop solution 10ml
    PLA-SEA Adhesive cover 4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mean(pg/ml) 374 1696 6130 451 1824 8468
    Standard deviation 17.95 79.71 300.37 23.90 98.50 567.36
    CV(%) 4.8% 4.7% 4.9% 5.3% 5.4% 6.7%

    Rat DNER PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20)
    Concentration(pg/ml) 0 312 625 1250 2500 5000 10000 20000
    O.D. 0.009 0.119 0.209 0.403 0.749 1.135 1.557 2.129
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

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