货号 | EK1889 |
---|---|
产品名称 | Rat CPE PicoKine ELISA Kit |
产品描述 | For quantitative detection of rat CPE in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, L43-F476 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | Cpe |
基因全名 | carboxypeptidase E |
Uniprot ID | P15087 |
指标别名 | Carboxypeptidase E; CPE; Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase |
背景资料 | CPE may play a role in post-translational processing of peptide hormones including preproopiomelanocortin. It is mapped to 16p13.This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1889-CAP | 96-well plate precoated with anti- rat CPE antibody | 1 |
EK1889-ST | Lyophilized recombinant rat CPE standard | 10ng/tube×2 |
EK1889-DA | Biotinylated anti- rat CPE antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 72 | 271 | 858 | 81 | 282 | 926 |
Standard deviation | 3.96 | 20.87 | 39.47 | 4.70 | 17.48 | 63.89 |
CV(%) | 5.5% | 7.7% | 4.6% | 5.8% | 6.2% | 6.9% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.013 | 0.068 | 0.119 | 0.202 | 0.409 | 0.787 | 1.459 | 2.189 |
典型操作程序:
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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