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Rat Chordin PicoKine ELISA Kit 96T

价:
2480.00
价:
¥2480.00

号:EK2086

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

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Product Brief

  • 产品概况

    货号 EK2086
    产品名称 Rat Chordin PicoKine ELISA Kit
    产品描述 For quantitative detection of rat Chordin in cell culture supernates, serum and plasma (heparin, EDTA).
    免疫原 NSO, T27-S948
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <50pg/ml
    检测范围 62.5pg/ml-4000pg/ml
    基因名称 Chrd
    基因全名 chordin
    Uniprot ID Q63148
    指标别名 Chordin; Chrd
    背景资料 Chordin is a bone morphogenetic protein antagonist composed of four small cysteine-rich domains, whose function is not known. In humans, the chordin peptide is encoded by the CHRD gene. It is mapped to 11q23. It dorsalizes the developing embryo by binding ventralizing TGFβ proteins such as bone morphogenetic proteins. It may also play a role in organogenesis. In mice, Chordin is expressed in the node but not in the anterior visceral endoderm. It has been found to be required for forebrain development. In developing mice that are deficient in both chordin and noggin, the head is nearly absent. This is significant because when only noggin is deficient there are mild defects but the head still forms. Chordin is also involved in avian gastrulation. It is expressed in the anterior cells of Koller's sickle, which form the anterior cells of the primitive streak, a key structure through which gastrulation occurs.
    显色时间 20-25

    试剂盒内容

    SKU 名称 数量
    Catalog number Description Quantity
    EK2086-CAP 96-well plate precoated with anti- rat Chordin antibody 1
    EK2086-ST Lyophilized recombinant rat Chordin standard 10ng/tube×2
    EK2086-DA Biotinylated anti- rat Chordin antibody 130μl(dilution 1:100)
    AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100)
    AR1106-1 Sample diluent buffer 30ml
    AR1106-2 Antibody diluent buffer 12ml
    AR1106-3 ABC diluent buffer 12ml
    AR1104 TMB color developing agent 10ml
    AR1105 TMB stop solution 10ml
    PLA-SEA Adhesive cover 4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mean(pg/ml) 95 293 1596 100 395 1791
    Standard deviation 4.85 15.53 89.38 6.60 26.86 127.16
    CV(%) 5.1% 5.3% 5.6% 6.6% 6.8% 7.1%

    Rat Chordin PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 20-25)
    Concentration(pg/ml) 0 62.5 125 250 500 1000 2000 4000
    O.D. 0.152 0.173 0.225 0.311 0.496 0.819 1.297 2.064
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

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