货号 | EK1699 |
---|---|
产品名称 | Rat Agrin PicoKine ELISA Kit |
产品描述 | For quantitative detection of rat Agrin in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | sf21,A1153-P1959 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | Agrn |
基因全名 | agrin |
Uniprot ID | P25304 |
指标别名 | Agrin; Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment; C90; Agrin C-terminal 22 kDa fragment; C22; Agrn; Agrin |
背景资料 | Agrin is a large proteoglycan whose best-characterised role is in the development of the neuromuscular junction during embryogenesis. Agrin is named based on its involvement in the aggregation of acetylcholine receptors during synaptogenesis. In humans, this protein is encoded by the AGRN gene.This protein has nine domains homologous to protease inhibitors.It may also have functions in other tissues and during other stages of development. It is a major proteoglycan component in the glomerular basement membrane and may play a role in the renal filtration and cell-matrix interactions. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1699-CAP | 96-well plate precoated with anti- rat Agrin antibody | 1 |
EK1699-ST | Lyophilized recombinant rat Agrin standard | 10ng/tubex2 |
EK1699-DA | Biotinylated anti- rat Agrin antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 57 | 219 | 767 | 60 | 223 | 776 |
Standard deviation | 2.39 | 10.73 | 39.1 | 3.36 | 14.27 | 50.1 |
CV(%) | 4.2% | 4.9% | 5.1% | 5.6% | 6.4% | 7.1% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.079 | 0.156 | 0.211 | 0.336 | 0.565 | 0.991 | 1.741 | 2.376 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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