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Mouse TNFRSF23 PicoKine ELISA Kit 96T

价:
2800.00
价:
¥2800.00

号:EK2008

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

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Product Brief

  • 产品概况

    货号 EK2008
    产品名称 Mouse TNFRSF23 PicoKine ELISA Kit
    产品描述 For quantitative detection of mouse TNFRSF23 in cell culture supernates, serum and plasma (heparin, EDTA, citrate).
    免疫原 NSO, M30-C155
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <10pg/ml
    检测范围 15.6pg/ml-1000pg/ml
    基因名称 Tnfrsf23
    基因全名 tumor necrosis factor receptor superfamily, member 23
    Uniprot ID Q9ER63
    指标别名 Tumor necrosis factor receptor superfamily member 23; Decoy TRAIL receptor 1; TNF receptor family member SOB; TNF receptor homolog 1; Tumor necrosis factor receptor p60 homolog 1; Tnfrsf23; Dctrailr1; Tnfrh1; Tnfrsf1al1
    背景资料 Mouse TNFRSF23, also known as TNFRH1 and DcTRAIL R1, is a GPI-anchored mouse TNF receptor superfamily member that lacks a cytoplasmic death domain and does not induce apoptosis. This gene is mapped to 7 F5; 7 88.28 Cm. This gene encodes a member of the tumor necrosis factor superfamily of proteins. The encoded receptor has been shown to bind to the ligand TRAIL (tumor necrosis factor-related apoptosis-inducing ligand), but to have no signaling capacity. This gene shows elevated expression in mice with diet-induced fatty liver disease. This gene and other family members are present in a gene cluster on chromosome 7. Alternative splicing results in multiple transcript variants.
    显色时间 15-20

    试剂盒内容

    SKU 名称 数量
    Catalog number Description Quantity
    EK2008-CAP 96-well plate precoated with anti- mouse TNFRSF23 antibody 1
    EK2008-ST Lyophilized recombinant mouse TNFRSF23 standard 10ng/tube×2
    EK2008-DA Biotinylated anti- mouse TNFRSF23 antibody 130μl(dilution 1:100)
    AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100)
    AR1106-1 Sample diluent buffer 30ml
    AR1106-2 Antibody diluent buffer 12ml
    AR1106-3 ABC diluent buffer 12ml
    AR1104 TMB color developing agent 10ml
    AR1105 TMB stop solution 10ml
    PLA-SEA Adhesive cover 4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mean(pg/ml) 31 118 452 33 124 468
    Standard deviation 1.49 5.55 22.15 1.68 6.70 31.36
    CV(%) 4.8% 4.7% 4.9% 5.1% 5.4% 6.7%

    Mouse TNFRSF23 PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20)
    Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000
    O.D. 0.067 0.111 0.147 0.231 0.391 0.700 1.345 2.226
    *典型数据仅供参考。用户需要自己建立标准曲线。

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