货号 | EK1629 |
---|---|
产品名称 | Mouse TFF3 PicoKine ELISA Kit |
产品描述 | For Quantitative Detection of mouse TFF3 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli,A23-F81 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | Tff3 |
基因全名 | trefoil factor 3, intestinal |
Uniprot ID | Q62395 |
指标别名 | Trefoil factor 3; Intestinal trefoil factor; mITF; Tff3; Itf |
背景资料 | Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1629-CAP | 96-well plate precoated with anti- mouse TFF3 antibody | 1 |
EK1629-ST | Lyophilized recombinant mouse TFF3 standard | 10ng/tube×2 |
EK1629-DA | Biotinylated anti- mouse TFF3 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 77 | 289 | 895 | 74 | 263 | 976 |
Standard deviation | 4.62 | 20.23 | 59.96 | 5.25 | 19.72 | 68.32 |
CV(%) | 6% | 7% | 6.7% | 7.1% | 7.5% | 7% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.048 | 0.127 | 0.203 | 0.365 | 0.624 | 1.001 | 1.667 | 2.501 |
典型操作程序:
1?准备试剂和标准品
2?每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3?每孔添加100ul生物素标记抗体,37℃反应60分钟。
4?0.01M TBS洗涤3次。
5?每孔添加100ulABC,37℃反应30分钟。
6?0.01M TBS洗涤5次。
7?TMB37℃避光反应30分钟以内。
8?加入TMB终止液,读数。
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