货号 | EK1936 |
---|---|
产品名称 | Mouse SMOC1 PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse SMOC1 in cell culture supernates, serum and plasma (heparin). |
免疫原 | NSO, H26-V463 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | Smoc1 |
基因全名 | SPARC related modular calcium binding 1 |
Uniprot ID | Q8BLY1 |
指标别名 | SPARC-related modular calcium-binding protein 1; SPARC-related gene protein; Secreted modular calcium-binding protein 1; SMOC-1; Smoc1; Srg |
背景资料 | This gene is mapped to 12; 12 D1. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1936-CAP | 96-well plate precoated with anti- mouse SMOC1 antibody | 1 |
EK1936-ST | Lyophilized recombinant mouse SMOC1 standard | 10ng/tube×2 |
EK1936-DA | Biotinylated anti- mouse SMOC1 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 59 | 214 | 919 | 60 | 231 | 947 |
Standard deviation | 3.07 | 13.48 | 44.11 | 2.94 | 17.09 | 50.19 |
CV(%) | 5.2% | 6.3% | 4.8% | 4.9% | 7.4% | 5.3% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.104 | 0.148 | 0.189 | 0.261 | 0.456 | 0.762 | 1.223 | 1.982 |
典型操作程序:
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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