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| 货号 | EK1996 |
|---|---|
| 产品名称 | Mouse SLPI PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of mouse SLPI in cell culture supernates, serum and plasma (heparin, EDTA). |
| 免疫原 | NSO, G26-M131 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 31.2pg/ml-2000pg/ml |
| 基因名称 | Slpi |
| 基因全名 | secretory leukocyte peptidase inhibitor |
| Uniprot ID | P97430 |
| 指标别名 | Antileukoproteinase; ALP; Secretory leukocyte protease inhibitor; Slpi |
| 背景资料 | Antileukoproteinase, also known as secretory leukocyte protease inhibitor (SLPI), is an enzyme that in humans is encoded by the SLPI gene. It is mapped to 2; 2 H3. SLPI is a highly cationic single-chain protein with eight intramolecular disulfide bonds. It is found in large quantities in bronchial, cervical, and nasal mucosa, saliva, and seminal fluids. SLPI inhibits human leukocyte elastase, human cathepsin G, human trypsin, neutrophil elastase, and mast cell chymase. X-ray crystallography has shown that SLPI has two homologous domains of 53 and 54 amino acids, one of which exhibits anti-protease activity (C-terminal domain). The other domain (N-terminal domain) is not known to have any function.This gene encodes a secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes; the protein is also thought to have broad-spectrum anti-biotic activity. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1996-CAP | 96-well plate precoated with anti- mouse SLPI antibody | 1 |
| EK1996-ST | Lyophilized recombinant mouse SLPI standard | 2ng/tube×2 |
| EK1996-DA | Biotinylated anti- mouse SLPI antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 59 | 214 | 938 | 62 | 237 | 958 |
| Standard deviation | 2.48 | 11.34 | 53.47 | 3.84 | 13.75 | 60.35 |
| CV(%) | 4.2% | 5.3% | 5.7% | 6.2% | 5.8% | 6.3% |
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.018 | 0.127 | 0.197 | 0.414 | 0.769 | 1.330 | 2.069 | 2.463 |
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