货号 | EK1885 |
---|---|
产品名称 | Mouse SerpinE2 PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse SerpinE2 in cell culture supernates, serum and plasma (heparin, EDTA, citrate) and urine. |
免疫原 | NSO, S20-P397 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <0.1ng/ml |
检测范围 | 0.78ng/ml-50ng/ml |
基因名称 | Serpine2 |
基因全名 | serine (or cysteine) peptidase inhibitor, clade E, member 2 |
Uniprot ID | Q07235 |
指标别名 | Glia-derived nexin; GDN; Peptidase inhibitor 7; PI-7; Protease nexin 1; PN-1; Protease nexin I; Serine protease-inhibitor 4; Serpin E2; Serpine2; Pi7; Pn1; Spi4 |
背景资料 | Glia-derived nexin is a protein that in humans is encoded by the SERPINE2 gene. This gene is mapped to 1 C4; 1 40.97 cM. SERPINE2 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 386 aminoacids (20-397a.a.) andhaving a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).SERPINE2 is expressed with a 8 aminoacid His tag atC-Terminus and purified by proprietary chromatographic techniques. This gene displays neurite promoting activity and serine protease inhibitory activity. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1885-CAP | 96-well plate precoated with anti- mouse SerpinE2 antibody | 1 |
EK1885-ST | Lyophilized recombinant mouse SerpinE2 standard | 50ng/tube×2 |
EK1885-DA | Biotinylated anti- mouse SerpinE2 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 599 | 2848 | 10128 | 604 | 2990 | 10197 |
Standard deviation | 33.54 | 165.18 | 455.76 | 36.84 | 191.36 | 723.99 |
CV(%) | 5.6% | 5.8% | 4.5% | 6.1% | 6.4% | 7.1% |
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.089 | 0.125 | 0.181 | 0.287 | 0.449 | 0.886 | 1.85 | 2.043 |
典型操作程序:
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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