试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK2056-CAP	96-well plate precoated with anti- mouse SEMA6A antibody	1
EK2056-ST	Lyophilized recombinant mouse SEMA6A standard	10ng/tube×2
EK2056-DA	Biotinylated anti- mouse SEMA6A antibody	130μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	130μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

• 1.96孔读板仪器。
• 2.自动洗板机.
• 3.移液器和枪头。建议使用多通道移液器/排枪。
• 4.Eppendorf 管。
• 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
  • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H₂O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
  • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na₂HPO₄ and 0.2g NaH₂PO₄ to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。