货号 | EK1694 |
---|---|
产品名称 | Mouse SCGB3A1 PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse SCGB3A1 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli,F22-G104 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <5pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | Scgb3a1 |
基因全名 | secretoglobin, family 3A, member 1 |
Uniprot ID | Q920D7 |
指标别名 | Secretoglobin family 3A member 1; Cytokine HIN-1; High in normal 1; Pneumo secretory protein 2; PnSP-2; Uteroglobin-related protein 2; Scgb3a1; Hin1; Pnsp2; Ugrp2 |
背景资料 | Secretoglobin family 3A member 1 is a protein that in humans is encoded by the SCGB3A1 gene. This gene encodes a small secreted protein that may function in the inhibition of cell growth. Expression of the related gene in humans is downregulated via DNA methylation in breast cancers and other tumor types. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1694-CAP | 96-well plate precoated with anti- mouse SCGB3A1 antibody | 1 |
EK1694-ST | Lyophilized recombinant mouse SCGB3A1 standard | 10ng/tubex2 |
EK1694-DA | Biotinylated anti- mouse SCGB3A1 antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 37 | 194 | 396 | 36 | 197 | 415 |
Standard deviation | 2.73 | 12.8 | 21.78 | 3.06 | 13.59 | 24.9 |
CV(%) | 7.4% | 6.6% | 5.5% | 8.5% | 6.9% | 6% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.099 | 0.164 | 0.220 | 0.342 | 0.556 | 0.994 | 1.631 | 2.253 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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