货号 | EK1635 |
---|---|
产品名称 | Mouse SCGB1A1/uteroglobin PicoKine ELISA Kit |
产品描述 | For Quantitative Detection of mouse SCGB1A1 in cell culture supernates, serum, plasma (heparin, EDTA) and urine. |
免疫原 | E.coli,D22-F96 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <1pg/ml |
检测范围 | 4.7pg/ml-300pg/ml |
基因名称 | Scgb1a1 |
基因全名 | secretoglobin, family 1A, member 1 (uteroglobin) |
Uniprot ID | Q06318 |
指标别名 | Uteroglobin; Clara cell 17 kDa protein; Clara cell phospholipid-binding protein; CCPBP; Clara cells 10 kDa secretory protein; CC10; PCB-binding protein; Secretoglobin family 1A member 1; Scgb1a1; Cc10; Ugb; Utg |
背景资料 | SCGB1A1 (secretoglobin family 1A member 1) also known as Uteroglobin is a protein that in humans is encoded by the SCGB1A1 gene. SCGB1A1 is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is specifically expressed in Clara cells in the lungs. Numerous studies demonstrated that UG is a multifunctional protein with antiinflammatory/ immunomodulatory properties. It inhibits soluble phospholipase A (2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, UG manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1635-CAP | 96-well plate precoated with anti- mouse SCGB1A1 antibody | 1 |
EK1635-ST | Lyophilized recombinant mouse SCGB1A1 standard | 10ng/tube×2 |
EK1635-DA | Biotinylated anti- mouse SCGB1A1 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 14 | 51 | 122 | 13 | 47 | 124 |
Standard deviation | 0.64 | 2.49 | 7.32 | 0.79 | 2.35 | 8.3 |
CV(%) | 4.6% | 4.9% | 6% | 6.1% | 5% | 6.7% |
Concentration(pg/ml) | 0 | 4.7 | 9.4 | 18.8 | 37.5 | 75 | 150 | 300 |
O.D. | 0.031 | 0.167 | 0.257 | 0.421 | 0.730 | 1.239 | 1.920 | 2.368 |
典型操作程序:
1?准备试剂和标准品
2?每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3?每孔添加100ul生物素标记抗体,37℃反应60分钟。
4?0.01M TBS洗涤3次。
5?每孔添加100ulABC,37℃反应30分钟。
6?0.01M TBS洗涤5次。
7?TMB37℃避光反应30分钟以内。
8?加入TMB终止液,读数。
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