货号 | EK1835 |
---|---|
产品名称 | Mouse REG3A PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse REG3A in cell culture supernates, serum and plasma (heparin). |
免疫原 | E.coli, E27-Q175 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | Reg3a |
基因全名 | regenerating islet-derived 3 alpha |
Uniprot ID | O09037 |
指标别名 | Regenerating islet-derived protein 3-alpha; REG-3-alpha; Islet of Langerhans regenerating protein 3; Lithostathine 3; Pancreatitis-associated protein 2; Regenerating islet-derived protein III-alpha; Reg III-alpha; Reg3a; Pap2 |
背景资料 | Regenerating islet-derived protein 3 alpha is a protein that in humans is encoded by the REG3A gene. It is mapped to 6 C3; 6 34.76 cM. This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. Multiple alternatively spliced transcript variants encoding the same protein have been described for this gene but the full length nature of some transcripts is not yet known. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1835-CAP | 96-well plate precoated with anti- mouse REG3A antibody | 1 |
EK1835-ST | Lyophilized recombinant mouse REG3A standard | 10ng/tube×2 |
EK1835-DA | Biotinylated anti- mouse REG3A antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 30 | 174 | 502 | 32 | 209 | 528 |
Standard deviation | 2.19 | 9.05 | 23.59 | 2.05 | 11.70 | 23.23 |
CV(%) | 7.3% | 5.2% | 4.7% | 6.4% | 5.6% | 4.4% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.020 | 0.132 | 0.258 | 0.432 | 0.753 | 1.095 | 1.607 | 2.229 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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