货号 | EK1829 |
---|---|
产品名称 | Mouse Plexin C1 PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse Plexin C1 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, A35-T950 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 93.8pg/ml-6000pg/ml |
基因名称 | Plxnc1 |
基因全名 | plexin C1 |
Uniprot ID | Q9QZC2 |
指标别名 | Plexin-C1; Virus-encoded semaphorin protein receptor; CD232; Plxnc1; Vespr |
背景资料 | A Plexin is a protein which acts as a receptor for Semaphorin family signaling proteins. This gene is mapped to 10; 10 C2. It is classically known for its expression on the surface of axon growth cones and involvement in signal transduction to steer axon growth away from the source of Semaphorin. Plexin also has implications in development of other body systems by activating GTPase enzymes to induce a number of intracellular biochemical changes leading to a variety of downstream effects. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1829-CAP | 96-well plate precoated with anti- mouse Plexin C1 antibody | 1 |
EK1829-ST | Lyophilized recombinant mouse Plexin C1 standard | 10ng/tube×2 |
EK1829-DA | Biotinylated anti- mouse Plexin C1 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 168 | 824 | 2614 | 172 | 830 | 2761 |
Standard deviation | 7.2 | 47.8 | 162.07 | 11.35 | 48.97 | 209.8 |
CV(%) | 4.3% | 5.8% | 6.2% | 6.6% | 5.9% | 7.6% |
Concentration(pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.066 | 0.166 | 0.190 | 0.301 | 0.617 | 1.038 | 1.642 | 2.421 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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