货号 | EK1882 |
---|---|
产品名称 | Mouse Plexin B2 PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse Plexin B2 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | CHO, L20-P1201 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 0.78ng/ml-50ng/ml |
基因名称 | Plxnb2 |
基因全名 | plexin B2 |
Uniprot ID | B2RXS4 |
指标别名 | Plexin-B2; Plxnb2 |
背景资料 | Plexin-B2 is a protein that in humans is encoded by the PLXNB2 gene. It is mapped to 15 E3; 15 44.68 cM. Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1882-CAP | 96-well plate precoated with anti- mouse Plexin B2 antibody | 1 |
EK1882-ST | Lyophilized recombinant mouse Plexin B2 standard | 50ng/tube×2 |
EK1882-DA | Biotinylated anti- mouse Plexin B2 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 29 | 192 | 447 | 31 | 200 | 469 |
Standard deviation | 1.54 | 10.94 | 28.61 | 1.49 | 14.20 | 32.36 |
CV(%) | 5.3% | 5.7% | 6.4% | 4.8% | 7.1% | 6.9% |
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.126 | 0.183 | 0.235 | 0.344 | 0.536 | 0.917 | 1.513 | 2.230 |
典型操作程序:
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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