货号 | EK1945 |
---|---|
产品名称 | Mouse PLA2G2A PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse PLA2G2A in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | sf21, N22-C146 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | Pla2g2a |
基因全名 | phospholipase A2, group IIA (platelets, synovial fluid) |
Uniprot ID | P31482 |
指标别名 | Phospholipase A2, membrane associated; Enhancing factor; EF; GIIC sPLA2; Group IIA phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2A; Pla2g2a |
背景资料 | Phospholipase A2, membrane associated is an enzyme that in humans is encoded by the PLA2G2A gene. It is mapped to 4 D3; 4 70.57 cM. Proteins belonging to the phospholipase A2 (PLA2) family hydrolyze phospholipids into sn2 fatty acids and lysophospholipids. They function in a variety of cellular processes, including the digestion of phospholipids and the production of molecules that induce inflammatory responses. This gene encodes a member of the group II class of secretory PLA2s. The secreted enzyme binds to heparin on the cell surface. Mutations in this gene increase the occurrence of intestinal polyps caused by a dominant mutation in the adenomatosis polyposis coli gene. A frameshift inactivates this gene product in some mouse strains including the strain of the reference genome, C57BL/6J, whereas a functional protein is produced in other strains. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1945-CAP | 96-well plate precoated with anti- mouse PLA2G2A antibody | 1 |
EK1945-ST | Lyophilized recombinant mouse PLA2G2A standard | 10ng/tube×2 |
EK1945-DA | Biotinylated anti- mouse PLA2G2A antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 56 | 234 | 938 | 58 | 242 | 951 |
Standard deviation | 2.69 | 13.34 | 43.15 | 2.96 | 14.28 | 60.86 |
CV(%) | 4.8% | 5.7% | 4.6% | 5.1% | 5.9% | 6.4% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.051 | 0.185 | 0.312 | 0.515 | 0.824 | 1.142 | 1.448 | 1.722 |
典型操作程序:
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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