货号 | EK1838 |
---|---|
产品名称 | Mouse LYPD3 PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse LYPD3 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, L33-S343 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 93.6pg/ml-6000pg/ml |
基因名称 | Lypd3 |
基因全名 | Ly6/Plaur domain containing 3 |
Uniprot ID | Q91YK8 |
指标别名 | Ly6/PLAUR domain-containing protein 3; GPI-anchored metastasis-associated protein C4.4A homolog; Lypd3; C4.4a |
背景资料 | Ly6/PLAUR domain-containing protein 3 is a protein that in humans is encoded by the LYPD3 gene. This gene is mapped to 7; 7 A3. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1838-CAP | 96-well plate precoated with anti- mouse LYPD3 antibody | 1 |
EK1838-ST | Lyophilized recombinant mouse LYPD3 standard | 10ng/tube×2 |
EK1838-DA | Biotinylated anti- mouse LYPD3 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 111 | 913 | 2985 | 169 | 920 | 2991 |
Standard deviation | 4.66 | 41.09 | 152.2 | 9.97 | 58.88 | 224.3 |
CV(%) | 4.2% | 4.5% | 5.1% | 5.9% | 6.4% | 7.5% |
Concentration(pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.041 | 0.126 | 0.194 | 0.331 | 0.605 | 1.025 | 1.498 | 2.340 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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