货号 | EK1840 |
---|---|
产品名称 | Mouse JAM-C/JAM3 PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse JAM-C/JAM3 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, E30-N241 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | Jam3 |
基因全名 | junction adhesion molecule 3 |
Uniprot ID | Q9D8B7 |
指标别名 | Junctional adhesion molecule C; JAM-C; JAM-2; Junctional adhesion molecule 3; JAM-3; Jam3 |
背景资料 | JAM3 belongs to the junctional adhesion molecule protein family and functions as a receptor for another member of this family. This gene is mapped to 9; 9 A4. JAM3 plays a part in cell-cell adhesion. The soluble form of JAM3 is a mediator of angiogenesis. JAM3 is a counter-receptor for ITGAM, mediating leukocyteplatelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). A mutation in an intron of the JAM3 gene is linked with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1840-CAP | 96-well plate precoated with anti- mouse JAM-C/JAM3 antibody | 1 |
EK1840-ST | Lyophilized recombinant mouse JAM-C/JAM3 standard | 10ng/tube×2 |
EK1840-DA | Biotinylated anti- mouse JAM-C/JAM3 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 58 | 270 | 884 | 61 | 279 | 991 |
Standard deviation | 2.90 | 14.04 | 39.78 | 2.81 | 17.86 | 74.33 |
CV(%) | 5.0% | 5.2% | 4.5% | 4.6% | 6.4% | 7.5% |
Concentration(pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.007 | 0.070 | 0.183 | 0.314 | 0.490 | 0.896 | 1.512 | 2.129 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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