货号 | EK1708 |
---|---|
产品名称 | Mouse HRG/HPRG PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse HRG in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO,L19-K525 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | Hrg |
基因全名 | histidine-rich glycoprotein |
Uniprot ID | Q9ESB3 |
指标别名 | Histidine-rich glycoprotein; Histidine-proline-rich glycoprotein; HPRG; Hrg |
背景资料 | HRG is a glycoprotein that in humans is encoded by the HRG gene. The HRG protein is produced in the liver, and it could also be synthesized by monocytes, macrophages, and megakaryocytes. It possesses a multi-domain structure, which makes it capable of binding to numerous ligands and modulating various biological processes including immunity, vascularization and coagulation. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1708-CAP | 96-well plate precoated with anti- mouse HRG antibody | 1 |
EK1708-ST | Lyophilized recombinant mouse HRG standard | 10ng/tubex2 |
EK1708-DA | Biotinylated anti- mouse HRG antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 312 | 1100 | 4259 | 314 | 1299 | 4301 |
Standard deviation | 12.79 | 49.5 | 264.06 | 17.27 | 75.34 | 326.88 |
CV(%) | 4.1% | 4.5% | 6.2% | 5.5% | 5.8% | 7.6% |
Concentration(pg/ml) | 0 | 156 | 313 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.025 | 0.082 | 0.115 | 0.197 | 0.452 | 0.801 | 1.432 | 2.144 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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