货号 | EK1715 |
---|---|
产品名称 | Mouse Glypican-2/GPC2 PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse GPC2 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO,H22-S556 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | Gpc2 |
基因全名 | glypican 2 (cerebroglycan) |
Uniprot ID | Q8BKV1 |
指标别名 | Glypican-2; Secreted glypican-2; Gpc2 |
背景资料 | Glypican 2 (GPC2), also known cerebroglycan, is a protein which in humans is encoded by the GPC2 gene.Cerebroglycan is a glycophosphatidylinositol-linked integral membrane heparan sulfate proteoglycan found in the developing nervous system. Cerebroglycan participates in cell adhesion and is thought to regulate the growth and guidance of axons. Cerebroglycan has especially high affinity for laminin-1 |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1715-CAP | 96-well plate precoated with anti- mouse GPC2 antibody | 1 |
EK1715-ST | Lyophilized recombinant mouse GPC2 standard | 10ng/tubex2 |
EK1715-DA | Biotinylated anti- mouse GPC2 antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 128 | 499 | 1231 | 130 | 501 | 1456 |
Standard deviation | 8.06 | 28.9 | 57.86 | 7.67 | 35.07 | 113.57 |
CV(%) | 6.3% | 5.8% | 4.7% | 5.9% | 7.0% | 7.8% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 | |
O.D. | 0.019 | 0.125 | 0.232 | 0.464 | 0.766 | 1.435 | 1.963 | 2.271 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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