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| 货号 | EK2073 |
|---|---|
| 产品名称 | Mouse GFRA2 PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of mouse GFRA2 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
| 免疫原 | NSO, S22-S443 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <50pg/ml |
| 检测范围 | 312pg/ml-20000pg/ml |
| 基因名称 | Gfra2 |
| 基因全名 | glial cell line derived neurotrophic factor family receptor alpha 2 |
| Uniprot ID | O08842 |
| 指标别名 | GDNF family receptor alpha-2; GDNF receptor alpha-2; GDNFR-alpha-2; GFR-alpha-2; GDNF receptor beta; GDNFR-beta; Neurturin receptor alpha; NRTNR-alpha; NTNR-alpha; TGF-beta-related neurotrophic factor receptor 2; Gfra2; Gdnfrb; Trnr2 |
| 背景资料 | GDNF family receptor alpha-2 (GFRα2), also known as the neurturin receptor, is a protein that in humans is encoded by the GFRA2 gene. It is mapped to 14; 14 D2. The GFRA2 protein is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor. It is part of the GDNF receptor family. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. They both bind the GFRA2 receptor. The receptor mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. GFRA2 (gene) has been shown to interact with GDNF. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK2073-CAP | 96-well plate precoated with anti- mouse GFRA2 antibody | 1 |
| EK2073-ST | Lyophilized recombinant mouse GFRA2 standard | 20ng/tube×2 |
| EK2073-DA | Biotinylated anti- mouse GFRA2 antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 536 | 2108 | 8768 | 582 | 2224 | 9685 |
| Standard deviation | 23.05 | 94.86 | 412.10 | 32.59 | 128.99 | 600.47 |
| CV(%) | 4.3% | 4.5% | 4.7% | 5.6% | 5.8% | 6.2% |
| Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.072 | 0.117 | 0.199 | 0.249 | 0.678 | 1.090 | 1.434 | 2.227 |
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