货号 | EK2084 |
---|---|
产品名称 | Mouse FGFR4 PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse FGFR4 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | 0 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 312pg/ml-20000pg/ml |
基因名称 | Fgfr4 |
基因全名 | fibroblast growth factor receptor 4 |
Uniprot ID | Q03142 |
指标别名 | Fibroblast growth factor receptor 4; FGFR-4; Protein-tyrosine kinase receptor MPK-11; CD334; Fgfr4; Fgfr-4; Mpk-11 |
背景资料 | Fibroblast growth factor receptor 4 is a protein that in humans is encoded by the FGFR4 gene. It is mapped to 13 B1; 13 29.8 cM. FGFR4 has also been designated as CD334 (cluster of differentiation 334). The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. The genomic organization of this gene, compared to members 1-3, encompasses 18 exons rather than 19 or 20. Although alternative splicing has been observed, there is no evidence that the C-terminal half of the IgIII domain of this protein varies between three alternate forms, as indicated for members 1-3. This particular family member preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2084-CAP | 96-well plate precoated with anti- mouse FGFR4 antibody | 1 |
EK2084-ST | Lyophilized recombinant mouse FGFR4 standard | 20ng/tube×2 |
EK2084-DA | Biotinylated anti- mouse FGFR4 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 326 | 1378 | 8230 | 429 | 1514 | 9488 |
Standard deviation | 14.02 | 70.28 | 427.96 | 23.60 | 89.33 | 607.23 |
CV(%) | 4.3% | 5.1% | 5.2% | 5.5% | 5.9% | 6.4% |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.002 | 0.046 | 0.104 | 0.234 | 0.517 | 0.931 | 1.402 | 1.938 |
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