货号 | EK1622 |
---|---|
产品名称 | Mouse FABP2 PicoKine ELISA Kit |
产品描述 | For Quantitative Detection of mouse FABP2 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli,A2-E132 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | Fabp2 |
基因全名 | fatty acid binding protein 2, intestinal |
Uniprot ID | P55050 |
指标别名 | Fatty acid-binding protein, intestinal; Fatty acid-binding protein 2; Intestinal-type fatty acid-binding protein; I-FABP; Fabp2; Fabpi |
背景资料 | Fatty acid-binding protein 2 (FABP2), also known as Intestinal-type fatty acid-binding protein (I-FABP) is a protein that in humans is encoded by the FABP2 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. Using a human cDNA probe for in situ hybridization studies, they regionalized the assignment to 4q28-q31. The intestinal form of FABP was mapped to mouse chromosome 3 between the amylase and alcohol dehydrogenase-3 loci. Also, they may be responsible in the modulation of cell growth and proliferation. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1622-CAP | 96-well plate precoated with anti- mouse FABP2 antibody | 1 |
EK1622-ST | Lyophilized recombinant mouse FABP2 standard | 10ng/tube×2 |
EK1622-DA | Biotinylated anti- mouse FABP2 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 75 | 265 | 861 | 69 | 288 | 910 |
Standard deviation | 5.92 | 13.78 | 38.74 | 5.93 | 17.56 | 56.42 |
CV(%) | 7.9% | 5.2% | 4.5% | 8.6% | 6.1% | 6.2% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.051 | 0.117 | 0.194 | 0.306 | 0.525 | 0.899 | 1.423 | 2.182 |
典型操作程序:
1?准备试剂和标准品
2?每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3?每孔添加100ul生物素标记抗体,37℃反应60分钟。
4?0.01M TBS洗涤3次。
5?每孔添加100ulABC,37℃反应30分钟。
6?0.01M TBS洗涤5次。
7?TMB37℃避光反应30分钟以内。
8?加入TMB终止液,读数。
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