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Qiagen 凯杰 Biosharp Omega

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Mouse ERAP1 PicoKine ELISA Kit 96T

价:
2800.00
价:
¥2800.00

号:EK2028

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

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Product Brief

  • 产品概况

    货号 EK2028
    产品名称 Mouse ERAP1 PicoKine ELISA Kit
    产品描述 For quantitative detection of mouse ERAP1 in cell culture supernates, serum and plasma (heparin, EDTA).
    免疫原 NSO, M1-L930
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <50pg/ml
    检测范围 312pg/ml-20000pg/ml
    基因名称 Erap1
    基因全名 endoplasmic reticulum aminopeptidase 1
    Uniprot ID Q9EQH2
    指标别名 Endoplasmic reticulum aminopeptidase 1; ARTS-1; Adipocyte-derived leucine aminopeptidase; A-LAP; Aminopeptidase PILS; Puromycin-insensitive leucyl-specific aminopeptidase; PILS-AP; VEGF-induced aminopeptidase; Erap1; Appils; Arts1
    背景资料 Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator, also known as endoplasmic reticulum aminopeptidase 1 (ARTS-1), is a protein which in humans is encoded by the ARTS-1 gene. This gene is mapped to 13; 13 C1. Endoplasmic reticulum amino peptidase 1 is active in the endoplasmic reticulum, which is involved in protein processing and transport. This protein is an aminopeptidase, which is an enzyme that cleaves peptides at the N-terminal into smaller fragments called amino acids.
    显色时间 15-20

    试剂盒内容

    SKU 名称 数量
    Catalog number Description Quantity
    EK2028-CAP 96-well plate precoated with anti- mouse ERAP1 antibody 1
    EK2028-ST Lyophilized recombinant mouse ERAP1 standard 20ng/tube×2
    EK2028-DA Biotinylated anti- mouse ERAP1 antibody 130μl(dilution 1:100)
    AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100)
    AR1106-1 Sample diluent buffer 30ml
    AR1106-2 Antibody diluent buffer 12ml
    AR1106-3 ABC diluent buffer 12ml
    AR1104 TMB color developing agent 10ml
    AR1105 TMB stop solution 10ml
    PLA-SEA Adhesive cover 4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mean(pg/ml) 505 1975 8963 2058 587 9289
    Standard deviation 29.29 118.50 412.30 125.54 40.50 678.10
    CV(%) 5.8% 6.0% 4.6% 6.1% 6.9% 7.3%

    Mouse ERAP1 PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20)
    Concentration(pg/ml) 0 312 625 1250 2500 5000 10000 20000
    O.D. 0.068 0.134 0.189 0.318 0.575 0.961 1.456 2.109
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

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