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Mouse EphA4 PicoKine ELISA Kit 96T

价:
2480.00
价:
¥2480.00

号:EK1799

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

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Product Brief

  • 产品概况

    货号 EK1799
    产品名称 Mouse EphA4 PicoKine ELISA Kit
    产品描述 For quantitative detection of mouse EphA4 in cell culture supernates, serum and plasma (heparin, EDTA).
    免疫原 NSO, V20-T547
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <10pg/ml
    检测范围 31.2pg/ml-2000pg/ml
    基因名称 Epha4
    基因全名 Eph receptor A4
    Uniprot ID Q03137
    指标别名 Ephrin type-A receptor 4 (EC:2.7.10.1); Tyrosine-protein kinase receptor MPK-3; Epha4; Tyrosine-protein kinase receptor SEK-1; Sek; Sek1
    背景资料 EPH receptor A4 (ephrin type-A receptor 4) is a protein that in humans is encoded by the EPHA4 gene. It is mapped to 1 C4; 1 39.55 cM. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
    显色时间 15-20

    试剂盒内容

    SKU 名称 数量
    Catalog number Description Quantity
    EK1799-CAP 96-well plate precoated with anti- mouse EphA4 antibody 1
    EK1799-ST Lyophilized recombinant mouse EphA4 standard 10ng/tube×2
    EK1799-DA Biotinylated anti- mouse EphA4 antibody 130μl(dilution 1:100)
    AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100)
    AR1106-1 Sample diluent buffer 30ml
    AR1106-2 Antibody diluent buffer 12ml
    AR1106-3 ABC diluent buffer 12ml
    AR1104 TMB color developing agent 10ml
    AR1105 TMB stop solution 10ml
    PLA-SEA Adhesive cover 4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mean(pg/ml) 35 199 734 46 208 857
    Standard deviation 2.03 11.94 33.76 2.81 14.35 62.56
    CV(%) 5.8% 6.0% 4.6% 6.1% 6.9% 7.3%

    Mouse EphA4 PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20)
    Concentration(pg/ml) 0 31.2 62.5 125 250 500 1000 2000
    O.D. 0.032 0.175 0.294 0.511 0.851 1.320 1.825 2.213
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

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